NaCl DNA Standards
We did a lab with DNA standards. We made one to one solutions of onion DNA that we precipitated and a 4% NaCl soln. We measured the amount of DNA using spectrophotometers. When we boiled the solution before measurement, the NaCl precipitated. This made the solution cloudy and the absorbancy readings inaccurate.
I'm wondering if there could be another solution that could be used to make the one to one solution, such as distilled water, or if the NaCl solution has a special role in ionization?
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...en get close enough to each other that they can precipitate. Without a positive ion of some kind (doesn't have to be Na+ necessarily, but it's cheap and it works) the negative charges will keep the DNA molecules apart. You ...