Cell Biology Homework

1. Digestion of the oligonucleotide, AGUACAA, by pancreatic RNase would yield what products?

2. A 5.5 kb, circular plasmid is cut with the following restriction endonucleases, producing the fragments listed. Construct a restriction map for this plasmid.

Endonuclease DNA fragments (kb)
Eco RI 5.5
Pst I 5.5
Bam HI 2.5 and 3.0
Eco RI and Bam HI 1.5 and 2.5
Eco RI and Pst I 0.5 and 5.0
Bam HI and Pst I 1.0, 2.0 and 2.5

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... phosphorous atom, while His 119 protonates the O-R leaving group.
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<br>2. The transition state is a 2'-3' cyclic intermediate. It is hyrdrolized by the reverse process where water replaces the leaving group. His 12 now acts as an acid and His 19 acts as a base. The result is hydrolyzed RNA and the enzyme in its original state.
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<br>This is figure 14-3 is from Biochemistry. Voet and Voet. 2nd addition.
<br>2. A 5.5 kb, circular plasmid is cut with the following restriction endonucleases, producing the fragments listed. Construct a restriction map for this plasmid.
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<br>Endonuclease DNA ...