Cytoplasmic membrane system
Autoradiography depends upon particles emitted from radioactive atoms striking a photographic emulsion that lies on top of the tissue section. When the emulsion is developed, the site where the particle struck the emulsion is developed, the site where the particle struck the emulsion appears as a silver grain, as in figure 8.3a. how do you think the thickness of the section might affect the resolution of the technique, that is the ability to locate the precise site in the cell where radioactivity is incorporated?© SolutionLibrary Inc. solutionlibary.com 9836dcf9d7 https://solutionlibrary.com/biology/cell-and-molecular-biology/cytoplasmic-membrane-system-2k1
...rporate radioactive sulfur (35S), nucleic acids will incorporate radioactive phosphate (32P) or thymidine (3H-Thymidine).
3. The cells are fix (killed but preserved intact) and prepared for development. This involves using special machines or lasers to slice your sample very thinly.
4. The preparation is overlayed with a thin film of photographic emulsion and left in the dark to develop for several days.
5. Just as light can develop film so can the energy emitted from the radioisotopes.
6. The position of the radioactivity in each cells is indicated by the position of the developed silver grains on the film.
There are two important properties that researchers like to maximize on their developed image one is intensity and the other resolution. Intensity refers to the brightness of the image. The more intense the image the less time it ...