A standard procedure in pufying tubulin is to extract the tissue on ice, then add GTP and warm the tissue extract to 37C. The warmed extract is then centrifuged at a speed insufficient to pellet average sized proteins, but sufficient to pellet large protein complexes. After centrifugation, the supernant is discarded, and the solution is agian warmed for a few minutes. Then centrifugation at the same speed described above is again performed. This process is repeated several times.
How would this processs result in purification of tubulin?
What contaminating proteins would likely co-purify with the tubulin?© SolutionLibrary Inc. solutionlibary.com 9836dcf9d7 https://solutionlibrary.com/biology/cell-and-molecular-biology/tubulin-7dqc
...away. In general most proteins are quite "sticky" and require repeated steps for purification. Purification therefore results due to its properties in comparison to most cellular proteins.
Contamination in protein purification is a major issue and one that is not easy to eliminate. Essentially any protein sharing the insolubility characteristics of tubulin will be isolated along with it and anything that normally binds to tubulin, especially covalently, will be dragged along ...