Factors Involved When Genetically Modifying A Mouse

Suppose you are given the task of genetically modifying a mouse so that its skin cells secrete a bitter tasting protein that will protect it from predation. What factors would you have to consider in order to achieve this goal? You only need to consider protein targeting, do not be concerned with how you would get the gene into the mouse or how you would control expression of the gene once it is in the mouse.

© SolutionLibrary Inc. solutionlibary.com 9836dcf9d7 https://solutionlibrary.com/biology/genetics/factors-involved-when-genetically-modifying-a-mouse-2gn

Solution Preview

...on sequences that form part of the protein and may or may not be cleaved before the protein is mature.

Protein is synthesized in the cytosolic compartment of the cell, at the ribosomes. Signal peptides are often on the amino terminal end of the protein, so that as the protein is being translated the correct trafficking mechanism is activated. The protein is then transported through the endoplasmic reticulum and Golgi apparatus, where it undergoes post-translational modifications (including cleavage of signal peptide) and is packaged into vesicles for appropriate subsequent localization. Different signal peptides specify different localizations (for example, extracellular secretion, or transport to another organelle within the cell).

In this case, the protein must be secreted; therefore the signal peptide must specify that it enters the secretory pathway.

The protein may already possess a correct signal peptide, depending on its origin; however it is likely that it will not. Therefore ...