Main differences between reproductive and therapeutic cloning
What is the difference between reproductive and therapeutic cloning? Name one limitation for each technique. Make sure to differentiate between pluripotent and totipotent embryonic stem cells as well as adult and embryonic stem cells.© SolutionLibrary Inc. solutionlibary.com 9836dcf9d7 https://solutionlibrary.com/biology/genetics/main-differences-between-reproductive-and-therapeutic-cloning-34m
...and transferring it into an enucleated egg (using microinjection technique). The egg is then transferred into a surrogate mother and develops into the fetus. Several limitations exist that can impair healthy development after birth (e.g. sheep Dolly) including telomere shortening problem. Briefly, the chromosome telomeres shorten after each DNA replication (in somatic cells) dues to the nature of lagging strand replication (Okazaki fragments). It is thought to be a contributing factor to aging, therefore the adult cell DNA that is transferred into an egg during reproductive cloning has already aged and contains shorter telomeres. The clone has therefore a potential to suffer from premature aging. The problem of replicating the ends of chromosomes is solved by a specialized enzyme (telomerase) that extends telomeres using an RNA template that is part of the telomerase. Telomerase enzyme is active during embryo formation (sexual reproduction) and in cancer cells.
Involves isolation of cells from ICM (see figure above) and culturing them in ...