PCR Process

If the denaturation step of PCR was omitted, what would happen to the PCR process?

How is PCR used to determine that a person is infected with a specific bacterium?

Now that we have PCR, why would we use culture techniques?

If a DNA polymerase other than the polymerase from Thermus aquaticus was used in the PCR process, what would happen?

List the stages of PCR and describe the function or purpose of each.

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...be specifically worried about ie MRSA in which case a specific PCR to confirm or not can be made within a few hours. In some other cases you may want to use less specific primers for PCR to put you in a ball park faster. For example you could design a series of primers that establish aerobic, anaerobic and antibiotic resistance sequences. This allows a simple direct approach for treatment without specifically identifying the microbe. Another reason not to completely remove culture is that PCR is not 100% and in some cases more than 1 strain of bacteria can present so the combination of the techniques may be required.

The vast majority of proteins are denatured and rendered inoperable by heat. The thermas aquaticus (or Taq) enzyme is essentially heat resistant and can survive the high temperatures need for PCR denaturation (Thank Dr. Mullis, inventor of PCR)

Read: http://en.wikipedia.org/wiki/Polymerase_chain_reaction where you will find the following

Initialization step: This step consists of heating the reaction to a temperature of 94-96°C (or 98°C if extremely ...