The requirements of enzyme-linked-immunoassay.

With the steady developments in the field of laboratory experiments done around the world and shift from radio-immuno-assay to ELISA, present some practical implications about ELISA.

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...riodate oxidation of the extensive carbohydrate group of horseraddishperoxidase produces aldehyde groups that will combine with the amino groups of immunoglobulins to produce Schiff base conjugates that can be stabilized by reduction with sodium borohydride.

Ideally enzyme substrates should be stable, safe, and ...